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Sigma
GenElute™ Plant Genomic DNA Miniprep Kit
G2N70 -
sufficient for 70 purifications
DOWNLOAD MSDS (PDF)Synonym: Gen Elute, Plant Genomic DNA Miniprep
Properties
| Related Categories | DNA & RNA Purification, Genomic DNA Purification, Plant Molecular Biology, Plant Nucleic Acid Purification, from Plant |
| usage | sufficient for 70 purifications |
Description
Application
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as restriction endonuclease digestions and PCR amplification.
Features and Benefits
• Less than 40 minutes from plant tissue to purified genomic DNA, including disruption in liquid nitrogen
• DNA yields of up to 20 μg per prep
• No RNase treatment required
• 40% more purifications per kit than leading supplier
Other Notes
For additional information, please see www.sigma-aldrich.com/gen
Principle
Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze died material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
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Documents
Certificate of Analysis
| G2N70 - Bulletin (311KB) |
| G2N70 - Datasheet (77 KB) |
| DNA and RNA Guide (841 KB ) |
| Technical Article Summer 06 (233 KB ) |
| Similar Products |
Safety Information
| Hazard Codes | Xn,N |
| Risk Statements | 20/21/22-32-52/53 |
| Safety Statements | 36/37-61 |
Technical information & documentation associated with this product is available in the Safety & Documentation tab.
References
Bennett MD, Leitch IJ., Nuclear DNA amounts in angiosperms: targets, trends and tomorrow. Ann. Bot. 107, 467-590, (2011) Abstract
Raissig MT, Baroux C, Grossniklaus U., Regulation and flexibility of genomic imprinting during seed development. Plant Cell 23, 16-26, (2011) Abstract
Schmitz RJ, Zhang X., High-throughput approaches for plant epigenomic studies. Curr. Opin. Plant Biol. 14, 130-136, (2011) Abstract
Sonah H, Deshmukh RK, Sharma A, et. al., Genome-wide distribution and organization of microsatellites in plants: an insight into marker development in brachypodium. PLoS ONE 6, e21298, (2011) Abstract
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